"Introduction to CRISPR Gene Editing Workflow"
An "Introduction to CRISPR Gene Editing Workflows" pre-conference workshop session:
Date: 18th February 2025.
Time: 2:00 PM - 5:30 PM
Participants: 40 Selected Participants only.
Venue: Seminar Hall, Centre for Drug Discovery and Development, Sathyabama Research Park, Sathyabama.
Dr. Kalpana Surendranath and Gene Editors
University of Westminster, London, UK.
CRISPR is a Nobel Prize-winning discovery and a revolutionary genetic tool with a wide range of applications, including curing diseases, developing new crops, producing biofuels, and enhancing sustainability measures. The double-strand breaks introduced by the CRISPR/Cas system are repaired by the double-strand DNA repair mechanisms. The introduction of excess CRISPR/Cas complexes into cells forces conditions that promote imprecise repair resulting in mutations, insertions, or deletions at the target site, leading to the knockout of target genes. This workshop offers a unique opportunity for researchers from diverse disciplines who are keen to apply CRISPR/Cas technology in their work. Participants will engage in a concise yet comprehensive journey through three key stages of the gene editing workflow:
- Overview of the CRISPR/Cas System - In this stage, participants will explore the mechanism and versatility of the CRISPR/Cas system, gaining a solid foundation for its applications in genome editing.
- Duplexing Guides for CRISPR Plasmid Construction - This stage focuses on designing and assembling guide RNAs for effective targeting, with hands-on experience in plasmid construction.
- Clonal Screens to Investigate Editing in Selected Human Chromosome Locations – In this stage, participant learn how to perform clonal screens to evaluate editing efficiency and investigate outcomes at specific genomic loci.